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| Synonym: | X-chromosome linked inhibitor of apoptosis |
| MDL number: | MFCD03702962 |
Description
| Analysis Note | The biological activity is measured by its ability to inhibit DEVD-AFC cleavage in cell extracts (activated by cytochrome c and dATP) by recombinant human caspase 3 or recombinant human caspase 7. |
| Physical form | Supplied as a 0.2 μm filtered solution in 25 mM Hepes, pH 8.0, 0.1 M sodium chloride, and 1 mM dithiothreitol (DTT). |
| Biochem/physiol Actions | XIAP, a member of the inhibitor of apoptosis (IAP) family, inhibits caspases. The BIR2 domain of XIAP inhibits caspase 3 and caspase 7 and the BIR3 domain inhibits caspase 9. |
Properties
| recombinant | expressed in Escherichia coli |
| assay | >95% (SDS-PAGE) |
| form | buffered aqueous solution |
| mol wt | mol wt 58 kDa by SDS-PAGE |
| Gene Information | human ... XIAP(331) |
| shipped in | dry ice |
| storage temp. | −20°C |
Safety
References
| reference | Deveraux, Q., et al. EMBO J. 17, 2215, (1998) |
| | Deveraux, Q.L. EMBO J. 18, 5242, (1999) |
| | Suzuki, Y. J. Biol. Chem. 276, 27058, (2001) |
